SCALE UP OF CYCLONE BIOREACTOR FOR THE PRODUCTION OF BIODEGRADABLE PLASTICS

Dadi Rusendi

Abstrak


The production of microbial poly-ß-hydroxybutyrate (PHB) was studied using
cyclone bioreactors at both the 1 L lab-scale and in 75 L Pilot Plant Scale (PPS) of
Pilot Plant scale. The Culture used for PHB production was Alcaligenes eutrophus
DSM 545, grown on a mineral salt medium limited by the supply of nitrogen. Two
different carbon sources were used: standard commercial glucose and hydrolyzed
potato processing waste. The levels of dissolved oxygen obtained in the PPS were
strongly depended on the location at which the air was introduced into the
reactor. However, with aeration balanced between two injection points and a
similar level of power input, 17 W/L, the PPS was able to provide at least as much
oxygen transfer capability as the lab-scale reactor. Under all condition tested, the
PHB accumulation by A. eutrophus was very high, in excess of 80% of the
biomass dry weight. The potato starch was readily hydrolyzed to fermentable
sugars, however, the economic feasibility of using this carbon sources requires
further evaluation.


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