CONSERVE EPITOPES OF INFLUENZA VIRUS INDUCE INNATE AND ADAPTIVE IMMUNE RESPONSES TO PRODUCE SPECIFIC ANTIBODY AGAINST M2E PROTEIN

Mansyur, I -, Soemitro, S -, Subroto, T -, Soedjanaatmadja, U.M.S. -

Abstrak


The existing vaccines against influenza are based on
the generation of neutralizing antibody primarily
directed against surface protein, haemagglutinin
(HA) and neuraminidase (NA). However, antigenic
drift and occasional shift of these two membrane
glycoproteins, HA and NA, make vaccine production
cumbersome and necessitate yearly revision of
the vaccine seed strains by the World Health
Organization. For these reasons, many investigators
have often tried to look at the possibility of generating
a universal vaccine useful against more than one
influenza strain. The objective of research was to
obtain an alternative antigen as vaccine candidate
for universal flu vaccination, instead of HA and NA
components. In this study, we use conserved epitope
M2e which is consist of three major component
such as N-terminal M2e2-24 (24 amino acids),transmembrane
(59 amino acids) and C-terminal (19
amino acids). We design two components of antigen,
linier and branched structures. The antigens then
formulated with aluminium hydroxide gel compared
to FCA/IFA adjuvant. These vaccines were tested
their immunogenicity, and the potency to mature the
dendritic cells for stimulating either CD8+ T cell or
antibody-mediated immune responses. The antibody
titre and the maturity of dendritic cell indicated by
cytokines concentration such as; IFN-ã, IL2 and IL4
were measured by ELISA test.The result of research
showed that the conserved epitope of Me2 2-16 when
incorporated with P25 protein from canine distemper
virus (linear structure) in alhydrogel adjuvant has
greater potential to produce anti-M2e antibodies
than in Freund adjuvant. Alhydrogel adjuvant had
a stronger effect than Freund adjuvant. Alhydrogel
also stimulate the release of IL-2 and IL-4.
Keywords: Epitopes, innate and adaptive immune
responses, M2e-protein, haemagglutinin (HA),
neuraminidase (NA), adjuvant.


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