before the freeze-thaw test test and there
was an abnormal distribution of data in the
2nd batch of smix 9:1 formula and smix 7:1
batch 3 on the data after the freeze-thaw test
with a significance value of 0.000 <0.05 so
that the data distribution was concluded
abnormal. The statistical test used was the
Wiloxon test to compare the effect of the
freeze-thaw test on the initial pH (cycle 0)
and final pH of the preparation (cycle 6) in
each formula. The Wiloxon test was used
because the data distribution was not
normal, the Wiloxon test results showed the
asymp value. Sig of 0.000 <0.05 so it can
be concluded that there is a significant
difference in the pH value of each formula
between before and after the freeze-thaw
test
5. Conclusions
Nanoemulsion preparation with the smix
9:1 formula is the most optimum formula,
which is to produce preparations with quite
good characteristics organoleptically and
give a light orange color appearance, clear,
distinctive smell with a pH value that met
the SNI standard 16-164399-1996 with pH
values ranging from 7.13 to 7.15 and based
on the centrifugation test gave stable results
and had particle size, polydispersity index
and zeta potential values, respectively, 22.9
± 9.4 nm, 0.435 and -21,4 mV and the value
of entrapment efficiency ranges from
93.87% to 94.32%. However, the
thermodynamic stability is not good
enough. This is indicated by the instability
of the preparation during the freeze-thaw
test with the results of changes in color,
transparency and changes in pH.
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