Ozonation Pretreatment Evaluation for Xylanase Crude Extract Production from Corncob under Solid-State Fermentation

Efri Mardawati, Surya Martha Pratiwi, Robi Andoyo, Tita Rialita, Mochamad Djali, Yana Cahyana, Een Sukarminah, In-In Hanidah, Imas Siti Setiasih


Xylanases are highly exploited enzyme in industries, including food and chemical industry. Xylanases can be utilized in catalyzing the endohydrolysis of 1,4-β-xylosidic linkages in xylan, lignocellulosic component to produce xylose-monomer. This research aims to optimize xylanase production from alternative substrate, corncob. Corncob contains 41.17% of hemicellulose, polymer of xylan. Xylanases are produced through solid state fermentation by Trichoderma viride. Ratio between substrate and moistening solution was 0.63 g/mL with fermentation temperature 32,8OC. Variables varied include incubation time and pretreatment (using autoclave, ozonation, combination of ozonation and autoclave, also without pretreatment). Xylanase activity was measured by DNS method using 1% of xylan as substrate standard. The result showed that the best incubation time is 36 h with 14403.8707 U/mg protein for specific xylanase activity by using autoclave as pretreatment.  Ozonation pretreatment process can increase the enzyme activity of xylanase.

Full Text:



Polizeli, M L T M, et al., et al. 2005 Xylanases from Fungi: Properties and Industrial Applications. Appl. Microbiol. Biotechnol, pp. 577-591

Bajaj, B K and Singh, N P. 2010. Production Xylanase from an Alkali Tolerant Streptomycess sp. 7b under Solid-State Fermentation, Its Purification, and Characterization, Appl. Biochem. Biotechnol., pp. 1804-1818

Vazquez, M J, et al. 2000. Xylooligosaccharides: Manufacture and Applications.. Trends in Food Science and Technology

Schultz-Jensen, N, et al., et al. 2011. Plasma-Assisted Pretreatment of Wheat Straw for Ethanol Production, Appl. Biochem. Biotechnol, pp. 1010-1023.

Cirakovic, J and Diner, B A. 2010. Ozone Treatment of Biomass to Enhance Enzymatic Saccharification. US20100159521 A1.

Willey, J M, Sherwood, L and Woolverton, C J. 2008. Prescott, Harley, and Klein’s Microbiology, McGraw-Hill Higher Education.

Souza-Corrêa, J A, et al., et al. 2013, Atmospheric Pressure Plasma Pretreatment of Sugarcane Bagasse: The Influence of Moisture in the Ozonation Process. Appl. Biochem. Biotechnol, pp. 104-116.

Dziugan, P, et al., et al. 2016. Ozonation as an Effective Way to Stabilize New Kinds of Fermentation Media Used in Biotechnological Production of Liquid Fuel Additives, Biotechnol. Biofuels.

Whistler, R L. 1950. Xylan, in: Cantor, C.S.H. and S.M. (Ed.), Advances in Carbohydrate Chemistry, Academic Press, pp. 269-290.

Wu, L, et al., et al. 2011. Low Temperature Alkali Pretreatment for Improving Enzymatic Digestibility of Sweet Sorghum Bagasse for Ethanol Production, Bioresour. Technol., pp. 4793-4799.

Whetten, R and Sederoff, R. 1995. Lignin Biosynthesis, Plant Cell, pp. 1001–1013.

İbanoǧlu, S. 2001. Influence of Tempering with Ozonated Water on The Selected Properties of Wheat Flour, Research Gate, pp. 345-350.

O’Donnell, C, et al., et al. 2012. Ozone in Food Processing.

Lacheur, R M Le and Glaze, W H. 1996. Reactions of Ozone and Hydroxyl Radicals with Serine, Env. Sci Technol, pp. 1072-1080.

Zhao, H, et al., et al. 2007. Leaf Senescence and Grain Filling Affected by Post-anthesis High Temperatures in Two Different Wheat Cultivars, Research Gate, pp. 149-158.

Lakshmi, G S, Bhargavi, P L and Prakasham, R S. 2011. Sustainable Bioprocess Evaluation for Xylanase Production by Isolated Aspergillus terreus and Aspergillus fumigatus under Solid-State Fermentation using Oil Palm Empty Fruit Bunch Fiber, Curr. Trends Biotechnol. Pharm, pp. 1434-1444.

Wolfrom, M L and Tipson, R S. 2014. Advances in Carbohydrate Chemistry, Academic Press.


  • There are currently no refbacks.