The effect of musa acuminata stem in increasing macrophage and neovascular cells of healing process

Maharani Laillyza Apriasari, Dewi Puspitasari, Retno Pudji Rahayu, Diah Savitri Ernawati

Abstract


Introduction: Musa acuminata stem has both of imunostimulatory and imunosuppressant effects that are influenced by their concentrations. The study aimed to analyze the effect of Mauli banana stem extract (EMBS) to increase the number of macrophage and neovascular cells in traumatic ulcer healing. Methods: Rattus norvegicus of male Wistar strain  were used  as model of traumatic ulcer. The left buccal mucous was biopsied, then used the histopathology method to find the number of macrophage and neovascular cells. Results: EMBS gel was effected when administered at a concentration of 37.5% because it significantly increased the number of macrophage and neovascular cells in traumatic ulcer healing on the 3rd day to 5th days. Conclusion:  It can be concluded that EMBS within concentration of 37.5% can accelerate ulcer healing through the increasing  number of macrophage and neovascular cells.


Keywords


Condensed tannin, macrophage, Musa acuminata, neovascular, terpenoid saponin.

Full Text:

PDF

References


CavalcanteI G.M, de PaulaI R.J.S, de SouzaII L.P, SousaIII F.B, MotaIII M.R.L, AlvesIII A.P.N.N, 2011. Experimental model of traumatic ulcer in the cheek mucosa of rats. Acta Cirúrgica Brasileira, 26 (3) : 299.

Laskaris G, 2005. Treatment of Oral Diseases : A Concise Textbook. Thieme, New York. P. 169

Regezi, Joseph A, J.J Sciuba, R.C.K Jourdan, 2015. Oral Pathology : Clinical Pathologic Correlations.5th Ed. Saunders, St Louis.P.23-26

Field A, Longman L, 2003.Tyldesley’s Oral Medicine, Ed 5th , Oxford, New York. P:51.

Apriasari M.L, 2012. The Management of Chronic Traumatic Ulcer in Oral Cavity. Majalah Kedokteran Gigi Dental Journal, 45 (2) : 68-72.

Apriasari M.L, Iskandar, Suhartono E, 2014. Kandungan Ekstrak Metanol Batang Pisang Mauli (Musa sp) 100%. International Journal of Bioscince, Biochemistry and Bioinformatics, 4 (2) : 110-115.

Apriasari M.L, Endariantari A, Oktavianty I.K, 2015. The Effect of 25% Mauli Banana Stem Extract Gel to increase the epithel thickness of wound healing process in oral mucosa. Majalah Kedokteran Gigi Dental Journal, 48 (3) : 150-153.

Apriasari M.L, Dachlan Y.P, Ernawati D.S, 2016. Effect of Musa acuminata Stem by Immunohistochemistry Test in Ulcer. Asian Journal of Biochemistry, 11 (3) : 135-141.

Albert B, Johnson A, Lewis J, Raff M, Robert K, Walter P, 2008. Moleculer Biology of The Cell. 5th Ed. Garland Science. United States of Amerika. P. 928-930.

Taher M, Majid F.A.A, Sarmidi M.R, 2006. A Proantocyanidin From Cinnamomum Zeylanicum Stimulates Phosphorylation of Insulin Receptor In 3T3-L1 Adipocytes. Jurnal Teknologi, 44(F) : 53-68

Besung I.N.K, 2009. Pegagan (Centella asiatica) Sebagai Alternatif Pencegahan Penyakit Infeksi Pada Ternak. Buletin Veteriner Udayana, 1 (2) : 61-67

Tsala D.E, Amadou D, Habtemariam S, 2013. Natural Wound Healing and Bioactive Natural Products. Phytopharmacology, 4 (3) : 532-560

James M.J, Zzomerdijk JCBM, 2004. Phosphatidylinositol 3-kinase and mTOR Signaling Pathway Regulate RNA Polymerase I Transcription in Response to IGF-1 and Nutrients. The Journal of Biological Chemistry , 279 (10) : 8911-8918.

Mori S, Nada S, Kimura H, Tajima S, Takahashi Y, 2014. The mTOR Pathway Controls Cell Proliferation by Regulating the FoxO3a Transcription Factor via SGK1 Kinase. PLoS ONE , 9 (2) : e88891.

Brancato S.K, Albina J.E, 2011. Mini Review : Wound Macrophages As Key Regulators of Repair Origin, Phenotype, and Function. The American Journal of Pathology, 178 (1) : 19-25.

Napetschnig J, Wu H, 2013. Molecular Basis of NF-κB Signaling. Annu. Rev. Biophys, 42 (19) : 1–19

Malek S, Chen Y, Huxford T, Ghosh G, 2001. IκBβ, but not IκBα, functions as a classical cytoplasmic inhibitor of NF-κB dimers by masking both NFκB nuclear localization sequences in resting cells. J.Biol. Chem. 276 (48) : 45225–35

Huang C, Jacobson K, Scaller M.D, 2004. A Role for JNK-Paxillin Signaling in Cell Migration. Cell Cycle, 3 (1) : 4-5.

Papa S, Zazzeroni F, Pham C.G, Bubici C, Franzoso G, 2004. Linking JNK signaling to NFkβ : a Key to Survival. Journal of Cell Science 117 (22) : 5197-5205.

Morgan M.J, Liu Z, 2011. Review : Crosstalk of Reactive Oxygen Species and NFkβ Signaling. Cell Research , 21 : 103-115.Larjava H, 2012. Oral Wound Healing ; Cell Biology and Clinical Management. Wiley Blackwell, West Sussex, UK.P. 43

Bedir E, Pugh N, Calis I, Pasco D.S, Khan I.A, 2000. Immunostimulatory Effects of Cycloartane-Type Triterpene Glycosides from Astragalus Species. Biol. Pharm.Bull, 23 (7) : 834-837.

Tonnesen M.G, feng X, Clarck R.A.F, 2000. Angiogenesis In Wound Healing. The Cosiety for Investigative Dermatology, 5 (1) : 40-45

Guo S, Dipietro L.A, 2010. Factors Affecting Wound Healing. J.Dent.Res, 89 (3) : 219-229.

Soni H, Singhai A.k, 2012. A Recent update of Botanical For Wound Healing Activity. International Reseach Journal of Pharamcy, 3 (7) : 1-6

Larjava H, 2012. Oral Wound Healing ; Cell Biology and Clinical Management. Wiley Blackwell, West Sussex, UK.P. 43

Brancato S.K, Albina J.E, 2011. Mini Review : Wound Macrophages As Key Regulators of Repair Origin, Phenotype, and Function. The American Journal of Pathology, 178 (1) : 19-25.

Majewska I, Darmach E.G, 2011. Proangiogenic activity of plant extracts in accelerating wound healing : a new face of old phytomedicines. Acta Biochimica Polonica , 58 (4) : 449–460.

Li K, Diao Y, Zhang H, Wang S, Zhang Z,Yu B, Huang S, Yang H, 2011. Tannin Extract From Immature of Terminalia chebula Fructus Retz Promote Cutaneous Wound Healing in Rats. BMC Complementary and Alternative Medicine, 86 (11) : 1-9




DOI: https://doi.org/10.24198/pjd.vol30no2.18324

Article metrics

Abstract views : 0 | views : 0

Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 Padjadjaran Journal of Dentistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 Visitor Stat

Creative Commons License
Padjadjaran Journal of Dentistry is licensed under a Creative Commons Attribution 4.0 International License