Antibacterial activity of Zingiber officinale roscoe extract as a potential root canal irrigation solution against Enterococcus faecalis

Rodiyah Azhar, Elin Julianti, Setiawan Natasasmita, Hendra Dian Adhita Dharsono

Abstract


Introduction: This study was aimed to determine and formulate antibacterial activity of Zingiber Officinale Roscoe (Z. officinale) extract against Enterococcus faecalis (E. faecalis) as a potential irrigating solution which is easily available and cost-effective. Methods:  Extract of Z. officinale Roscoe was yielded through soxhlet technique. Samples divided into two groups, i.e., the extract of Z. officinale Roscoe and chlorhexidine 2% as a control. The antimicrobial activity was observed using the diffuse agar method. Minimum inhibitory concentration (MIC) was determined by serial microdilution method, and minimum bactericidal concentration (MBC) was determined by cultured samples in blood agar. Data were analysed using ANOVA and Kruskal-Wallis test. Results: Extract of Zingiber officinale Roscoe has antimicrobial activity against E. faecalis with the MBC of 15.625 mg/mL. (p<0,05). Conclusion: Extract of Zingiber officinale Roscoe have shown antibacterial activity against E. faecalis and can be further developed as a potential root canal irrigation solution.


Keywords


Antibacterial activity, Enterococcus faecalis, extract of Zingiber officinale Roscoe, root canal irrigating solution.

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Sjögren U, Figdor D, Persson S, Sundqvist G. Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Intern Endod J 1997; 30: 297–306.

Hasselgren G, Olsson B, Cvek M. Effects of calcium hydroxide and sodium hypochlorite on the dissolution of necrotic porcine muscle tissue. J Endod 1988; 14: 125–7.

Kovac J, Kovac D. Effect of irrigating solutions in endodontic therapy. Bratisl Lek Listy. 2011; 7: 410-415.

Pujar M, Makandar S. Herbal usage in endodontics- A review. Intern J Contemp Dent 2011; 2: 34-37.

Demin G, Yingying, Z (2010) Comparative antibacterial activities of crude polysaccharides and flavonoids from Zingiber officinale and their extraction. American Journal of Tropical Medicine 5: 235-238.

Sasidharan I, Nirmala Menon A (2010) Comparative Chemical Composition and Antimicrobial Activity Fresh & Dry Ginger Oils (Zigiber Officinale Roscoe).International Journal of Current Pharmaceutical Research 2: 40-43.

Sebiomo A, Awofodu AD, Awosanya AO, Awotona FE, Ajayi AJ (2011) Comparative studies of antibacterial effect of some antibiotics and ginger (Zingiber officinale) on two pathogenic bacteria. Journal of Microbiology and Antimicrobials 3: 18-22.

Lee YB, Kim YS, Ahmore CR (1986) Antioxidant property in ginger rhizoma and its application to mate products. Journal of Food Science 51: 20-23.

Penna SC, Medeiros MV, Aimbire FS, Faria-Neto HC, Sertié JA, et al. (2003) Anti-inflammatory effect of the hydralcoholic extract of Zingiber officinale rhizomes on rat paws and skin edema. Phytomedicine 10: 381-385.

Kadnur SV, Goyal RK (2005) Beneficial effects of Zingiber officinale Roscoe on fructose induced hyperlipidemia and hyperinsulinemia in rats. Indian J Exp Biol 43: 1161-1164.

Islam MS, Choi H (2008) Comparative effects of dietary ginger (Zingiber officinale) and garlic (Allium sativum) investigated in a type 2 diabetes model of rats. J Med Food 11: 152-159.

Kim JS, Lee SI, Park HW, Yang JH, Shin TY, et al. (2008) Cytotoxic components from the dried rhizomes of Zingiber officinale Roscoe. Arch Pharm Res 31: 415-418.

Isa Y, Miyakawa Y, Yanagisawa M, Goto T, Kang MS, et al. (2008) 6-Shogaol and 6-gingerol, the pungent of ginger, inhibit TNF-a mediated down regulation of adiponectin expression via different mechanisms in 3T3-L1 adipocytes. Biochem and BiophyS ReS Commun 373: 429–434.

Wang W, Li CY, Wen XD, Li P, Qi LW (2009) Simultaneous determination of 6-gingerol, 8-gingerol, 10-gingerol and 6-shogaol in rat plasma by liquid chromatography–mass spectrometry: Application to pharmacokinetics. J Chromatogr B Analyt Technol Biomed Life Sci B 877: 671–679.

Shim S, Kim S, Choi DS, Kwon YB, Kwon J (2011) Anti-inflammatory effects of [6]-shogaol: Potential roles of HDAC inhibition and HSP70 induction. Food Chem Toxicol 49: 2734–2740.

Park M, Bae J, Lee DS. Antibacterial Activity of [10] Gingerol and [12]-Gingerol isolated from Ginger Rhizome against Periodontal Bacteria. Phytother Res 2008; 22: 1446–1449.

Sebiomo A, Awofodu AD, Awosanya AO, Awotona FE, Ajayi AJ. Comparative studies of antibacterial effect of some antibiotics and ginger (Zingiber officinale) on two pathogenic bacteria. J Microbiol Antimicrob. 2011; 3: 18-22.

Atai Z, Atapour M, Mohseni M. Inhibitory Effect of Ginger Extract on Candida albicans. Am J Appl Sci. 2009; 6: 1067-1069.

Gulve MN, Gulve ND. Comparison of Antimicrobial Efficacy of Ginger Extract and 2% Sodium Hypochlorite against Enterococcus faecalis using agar diffusion method. J Ind Dent Assoc 2010; 4: 347-349.

Lin S, Zuckerman O, Wiess EI, Mazor Y, Fuss Z. Antibacterial efficacy of a new Chlorohexidine slow release device to disinfect tubules. J Endod 2003; 29: 416-418.

Jeansonne MJ, White RR. A comparison of 2% Chlorohexidine glugonate and 5.25% sodium hypochlorite as antimicrobial endodontic irrigant. J Endod 1994; 20: 276-278.




DOI: https://doi.org/10.24198/pjd.vol30no2.18328

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