Pengaruh aplikasi bovine amniotic membrane pada soket tulang alveolar terhadap ekspresi BMP-2: studi eksperimental in vitro

Geneva Ariesta; Octarina Octarina; Elly Munadziroh; Ekowati Handharyani

doi:10.24198/jkg.v35i2.46718

Pengaruh aplikasi bovine amniotic membrane pada soket tulang alveolar terhadap ekspresi BMP-2: studi eksperimental in vitro

Geneva Ariesta, Octarina Octarina, Elly Munadziroh, Ekowati Handharyani

Abstract

ABSTRAK

Pendahuluan: Pencabutan gigi dapat menyebabkan resorpsi tulang alveolar. Bovine Amniotic Membrane (BAM) diharapkan menjadi material pengisi soket tulang alveolar sehingga dapat mengurangi terjadinya resorpsi tulang alveolar setelah dilakukan pencabutan. BMP-2 merupakan indikator terjadinya pembentukan tulang alveolar. Tujuan penelitian ini adalah menganalisis ekspresi BMP-2 dengan aplikasi BAM pada soket tulang alveolar tikus Sprague dawley (SD). Metode: …….. …….. Jenis penelitan ini adalah eksperemintal laboratorik. Teknik pengambilan jumlah sampel menggunakan rumus Federer. Sebanyak 16 tikus Sprague dawley jantan dibagi menjadi dua kelompok, yaitu kelompok kontrol dan kelompok BAM. Pencabutan dilakukan pada gigi insisivus sentral kanan mandibula. Setelah dilakukan pencabutan, pada kelompok bam soket alveolar diaplikasikan material BAM dengan ukuran 1,5x10 mm. Hari ke-14 dan ke-28, euthanasia dilakukan, kemudian dilakukan pemotongan pada bagian soket tulang alveolar yang akan dipreparasi menjadi preparat imunohistokimia (IHK). Pembuatan preparat IHK menggunakan pewarnaan antigen-antibodi BMP-2 (1:200, Affinity Biosciences, Inc. USA). Analisis preparat IHK menggunakan mikroskop ZEISS seri AXIO SCOPE A.1 dan software ZEN 3.4 perbesaran 40x. Perhitungan ekspresi BMP-2 menggunakan software imageJ. Analisis data statistik penelitian menggunakan one-way ANOVA dengan uji post hoc Tukey. Hasil: Nilai rerata ekspresi BMP-2 pada kelompok kontrol hari ke-14 dan ke 28 secara berurutan (5,93 ± 0,70) dan (6,60 ± 1,11). Kelompok BAM menunjukkan adanya peningkatan nilai rerata ekspresi BMP-2 pada hari ke-14 dan ke-28 secara berurutan (9,46 ± 0,50) dan (11,73 ± 3,60) secara statistik dengan one way annova pos hoc tukey berbeda bermakna (p<0,05). Simpulan: Aplikasi material BAM pada soket tulang alveolar dapat meningkatkan ekspresi BMP-2 pada hari ke-14 dan 28. Hal tersebut akan menyebabkan penyembuhan tulang alveolar dan berkurangnya resorpsi tulang alveolar.

The effect of bovine amniotic membrane application at the alveolar bone socket on the expression of BMP-2: in vitro experimental study

ABSTRACT

Introduction: Tooth extraction can cause alveolar bone resorption. Bovine Amniotic Membrane (BAM) is expected to be a filling material for the alveolar bone socket so that it can reduce the occurrence of alveolar bone resorption after extraction. BMP-2 is an indicator of alveolar bone formation. The application of BAM is expected to reduce of alveolar bone resorption after extraction. Aim: The aim of this study was to analyze the expression of BMP-2 by application of BAM in the alveolar bone socket of Sprague Dawley rats (SD). Methods: This type of research is laboratory experimental. The sampling technique used the Federer formula. A total of 16 male SD rats were divided into two groups, which are the control group and the BAM group. Extraction was performed on the mandibular right central incisor. In the BAM group, after removal of the alveolar socket BAM material with a size of 1.5 x 10 mm was applied. On the 14th and 28th day, mice were euthanized and then cut into the alveolar bone socket which will be prepped into an immunohistochemical specimen (IHK). Preparation of IHK will be prepped using BMP-2 antigen-antibody staining (1:200, Affinity Biosciences, Inc. USA). Analysis of CPI preparate using the ZEISS AXIO SCOPE A.1 series microscope and ZEN 3.4 software with 40x magnification. BMP-2 expression calculation using imageJ software. Statistical data analysis of the research used one-way ANOVA with Tukey's post hoc test. Results: The mean value of BMP-2 expression in the control group on day 14 and 28 respectively (5.93 ± 0.70) and (6.60 ± 1.11). The BAM group showed an increase in the mean value of BMP-2 expression on the 14th and 28th days respectively (9.46 ± 0.50) and (11.73 ± 3.60) which were statistically significantly different (p<0, 05). Conclusion: Application of BAM to the alveolar bone socket can increase BMP-2 expression on days 14 and 28. This will lead to alveolar bone healing and reduced alveolar bone resorption.

Key words

bovine amnion membrane, bone morphogenetic protein 2, alveolar socket preservation

Keywords

bovine amniotic membrane, bone morphogenetic protein 2, preservasi soket tulang alveolar, bovine amnion membrane, bone morphogenetic protein 2, alveolar socket preservation

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References

DAFTAR PUSTAKA

Tal H, Artzi Z, Kolerman R, Beitlitum I, Goshen G. Augmentation and preservation of the alveolar process and alveolar ridge of bone. Research Gate. 2016;2(6):120-29. DOI:10.5772/33839

Farmer M, Darby I. Ridge dimensional changes following single-tooth extraction in the aesthetic zone. Clin Oral Impl Res. 2014;25(2):272-277. DOI: 10.1111/clr.12108

Hamed MT, Mously HA. Investigating the causes and effects of alveolar bone loss and the impact of restoration types; A systematic review analysis. Internat J Pharmaceut Res Allied Scie. 2019;8(4):60-70.

Viet Dam D, Trinh AH, Rokaya D, Trinh DH. Bone augmentation for implant placement: recent advances. Hindawi Internat J Dentis. 2022;940:1-7. DOI: 10.1155/2022/8900940

Sukuraman P, Dionysius D, Ngeow W, Tan CC, Hussin MZ. Systemic medications and implant success: J Dent Impl. 2022;12(2):78–85. DOI:10.4103/jdi.jdi_24_21

L Fee. Socket preservation. Br Dent J. 2017;222(8):576-82. DOI: 10.1038/sj.bdj.2017.355

Suchetha A, Tanwar E, Sapna N, Bhat D , Spandana A. Alveolar Bone in Health. Internat J Periodon Implant. 2017;2(4):112-116.

Araújo-Gomes N, Romero-Gavilán F, García-Arnáez I, Martínez-Ramos C, Sánchez-Pérez AM, Azkargorta M, et al. Osseointegration mechanisms: A proteomic approach. J Biologic Inorg Chemis. 2018;23(3):459–470. DOI: 10.1007/s00775-018-1553-9

Tang K, Wu J, Xiong Z, Ji Y, Sun T. Human acellular amniotic membrane: A potential osteoinductive biomaterial for bone regeneration. J. Biomater. Appl. 2017; 32: 754–764. DOI: 10.1177/0885328217739753

Octarina, Munadziroh E. Potential of bovine amniotic membrane and hydroxyapatite as biocomposite materials for enhanced bone formation. Mal J Med Health Sci. 2021;17:124-126.

Octarina, Munadziroh E, Abdel Razak F. Physical modification of bovine amniontic membrane for dental application. J Internat Dent Med Res. 2021;4:1425-1428.

Srinivas B, Das P, Rana M, Qureshi AQ, Vaidya KC, Raziuddin SJA. Wound healing and bone regeneration in postextraction sockets with and without platelet-rich fibrin. Amsjournal. 2018;8(1):28-34. DOI: 10.4103/ams.ams_153_17

Gonzalez AC, Costa T Andrade ZA, Medrado AR. Wound healing - A literature review. An Bras Dermatol. 2016; 91(5):614-620. DOI: 10.1590/abd1806-4841.20164741

Wang W, Yeung KW, Bone grafts and biomaterials substitutes for bone defect repair: a review. Bioactive Materials. 2017; 2: 224-247. DOI: 10.1016/j.bioactmat.2017.05.007

Sabouri L, Farzin A, Kabiri A, Milan PB, Farahbakhsh M, Mehdizadehkashi A, et al. Mineralized Human Amniotic Membrane as A Biomimetic Scaffold for Hard Tissue Engineering Applications. ACS Biomaterials Science and Engineering. 2020;6(11):6285–98. DOI: 10.1021/acsbiomaterials.0c00881

Araújo-Gomes N, Romero-Gavilán F, García-Arnáez I, Martínez-Ramos C, Sánchez-Pérez AM, Azkargorta M, et al. Osseointegration Mechanisms: A Proteomic Approach. Journal of Biological Inorganic Chemistry. 2018; 23(3): 459–470. DOI: 10.1007/s00775-018-1553-9

Ha SW, Park J, HabiB MM, Beck GR. Nano-hydroxyapatite stimulation of gene expression requires fgf receptor, phosphate transporter, and Erk1/2 signaling. ACS Appl. Mater. Interfaces. 2017; 9:39185–39196. DOI: 10.1021/acsami.7b12029

Wang J, Wang M, Chen F, Wei Y, Chen X, Zhou Y, et al. Nano-Hydroxyapatite Coating Promotes Porous Calcium Phosphate Ceramic-Induced Osteogenesis Via BMP/Smad Signaling Pathway. Internat J Nanomed. 2019;14:7987–8000. DOI: 10.2147/ijn.s216182

Bathla S, Damle S. Textbook of Periodontics. 1st ed. New Delhi: Jaypee Brothers Medical Pub; 2017. p. 37–39.

Indrawati DW, Munadziroh E, Indah T, Sulisetyawati B, Mahardhika P, Fadhlallah E. Sponge amnion potential in post tooth extraction wound healing by interleukin-6 and bone morphogenetic protein-2 expression analysis: an animal study. Dent Res J (Isfahan). 2019;16:283-8. DOI:10.4103/1735-3327.266089

Shin YP, Kyoung HK, Sungtae K, Yong ML, Yang JS. BMP-2 gene delivery-based bone regeneration in dentistry. Pharmaceutics. 2019;11:39. DOI: 10.3390/pharmaceutics11080393

Politis C, Schoenaers C, Jacobs R, Agbaje JO. Wound Healing Problems in The Mouth. Frontiers in Physiology. 2016; 7: 507. DOI: 10.3389/fphys.2016.00507

Rakian A, Wu CY, Gluhak-Heinrich J, Cui Y, Harris MA, Villarreal D, et al. Bone Morphogenetic protein-2 gene controls tooth root development in coordination with formation of the periodontium. Internat J Oral Scie. 2013;5:75–84. doi: 10.1038/ijos.2013.41

Tannoury CA, An HS. Complications with The Use of Bone Morphogenetic Protein 2 (BMP-2) in Spine Surgery. Spine J. 2014;14:552–559. DOI: 10.1016/j.spinee.2013.08.060

Gomez-Puerto MC, Iyengar PV, García de Vinuesa A, Ten Dijke P, Sanchez-Duffhues G, Bone Morphogenetic Protein Receptor Signal Transduction in Human Disease. J. Pathol. 2019;247:9–20. DOI: 10.1002/path.5170

Young DC, Kyoung HK, Yong ML, Young K, Yang JS. Periodontal wound healing and tissue regeneration: A narrative review. Pharmaceuticals. 2021;14:456. DOI: 10.3390/ph14050456

Jovanovic SA, Hunt DR, Bernard GW, Spiekermann H, Nishimura R, Wozney JM, et al. Long-term functional loading of dental implants in rhBMP-2 induced bone. A histologic study in the canine ridge augmentation model. Clin Oral Implants Res 2013; 14: 793-803. DOI: 10.1046/j.0905-7161.2003.clr140617.x

DOI: https://doi.org/10.24198/jkg.v35i2.46718