The inhibitory effect of galo-galo honey extract (heterotrigona itama) against pseudomonas aeruginosa: experimental study

Ria edelwis zwespa, Dina Auliya Amly, Widya Puspita Sari

Abstract


Introduction: Pseudomonas aeruginosa is a pathogenic bacterium and a major contributing factor in nosocomial infections. Although chlorhexidine mouthwash is widely used to prevent cross-infection, its improper use can cause adverse effects. Galo-galo bee honey (Heterotrigona itama) contains various antibacterial properties, including carbohydrates, proteins, phenolics and flavonoids. The purpose of this study was to analyze the effect of Galo-galo bee honey extract in inhibiting the growth of P. aeruginosa. Methods: This in vitro laboratory experiment evaluated the antibacterial activity of Galo-galo bee honey extract obtained via centrifugation. The extract was tested against P. Aeruginosa at concentrations of 0.09%, 0.14%, 0.39%, 0.78%, 1.56%, 3.13%, 6.25%, 12.5%, 25% and 50% to Results: The Galo-galo bees honey extract significantly inhibited the growth of P. aeruginosa at higher concentrations, yielding 73% at a 12.5 % concentration, 74% at 25%,  and 77% at 50%. Conversely, concentrations ranging from 0.09%to 6.25% showed no inhibitory effect against the bacteria. Conclusion: Galo-galo bee honey extract exhibits concentration-dependent antibacterial activity against P. Aeruginosa, with its   inhibitory effects


Efek ekstrak madu lebah galo-galo (Heterotrigona itama) dalam menghambat pertumbuhan Pseudomonas aeruginosa: studi eksperimental


Pendahuluan: Pseudomonas aeruginosa adalah bakteri patogen yang berperan utama dalam infeksi nosokomial. Penggunaan obat kumur klorheksidin untuk menghindari infeksi silang telah dilakukan, namun penggunaan yang tidak tepat dapat menimbulkan efek samping. Madu lebah Galo-galo (Heterotrigona itama) memiliki berbagai kandungan antibakteri, yaitu karbohidrat, protein, fenolik dan flavonoid. Tujuan penelitian menganalisis efek  ekstrak madu lebah Galo-galo (Heterotrigona itama) dalam menghambat pertumbuhan Pseudomonas aeruginosa. Metode: Penelitian ini dilakukan dengan metode eksperimental melalui pengujian di Laboratorium. Ekstrak madu lebah Galo-galo (Heterotrigon itama) yang diperoleh dengan cara sentrifugasi diuji dengan konsentrasi 0,09%, 0,19%, 0,39%, 0,78%, 1,56%, 3,13%, 6,25%, 12,5%, 25% dan 50% untuk melihat aktivitas antibakterinya terhadap bakteri P. aeruginosa. Hasil: Penelitian ini menunjukkan bahwa ekstrak madu lebah Galo-galo (Heterotrigona itama) menghambat pertumbuhan P. aeruginosa dengan persentase sebagai berikut: konsentrasi 12,5% (73%), 25% (74%), dan 50% (77%). Sedangkan pada konsentrasi 0,09%, 0,19%, 0,39%, 0,78%, 1,56%, 3,13% dan 6,25% ekstrak madu lebah Galo-galo (Heterotrgona itama)belum dapat menghambat pertumbuhan bakteri Pseudomonas aeruginosa, Simpulan: Efek ekstrak madu lebah Galo-galo (Heterotrigona itama) menunjukkan perbedaan dalam menghambat pertumbuhan P. aeruginosa dan daya hambat meningkat seiring dengan peningkatan konsentrasi madu




Keywords


antibacterial, heterotrigona itama, pseudomonas aeruginosa

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References


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DOI: https://doi.org/10.24198/jkg.v28i1.61757

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