Antibacterial potential of Biduri leaf extract (Calotropis gigantea) against the growth of Streptococcus mutans ATCC 35668 colonies: an experimental laboratory
Abstract
ABSTRACT
Introduction: Caries is an oral cavity infection that attacks the hard tissue of the teeth. Caries are caused by cariogenic bacteria such as Streptococcus mutans (S.mutans). S.mutans plays a role in the process of biofilm and plaque build up. One alternative ingredient that has antibacterial properties is Biduri leaves (Calotropis gigantea). The research aimed to analyze the antibacterial potential of Biduri leaf extract on the growth of S.mutans colonies. Methods: This type of research is a laboratory experimental research in vitro using the post-test-only control group design method. The antibacterial test used the colony counting method. The extraction process used the maceration method for 1 day with a shaker. The concentrations used were concentrations of 20, 40, and 60%. Control (+) used 0.2% chlorhexidine and control (-) used distilled water. Incubation process was for 24 hours. Counting the growth of S.mutans colonies using a colony counter was carried out by three observers. Data analysis used the Kruskal-Wallis test followed by the Mann-Whitney test. Results: The results showed that there was a significant difference in the growth of S.mutans (p<0.05) after being exposed to Biduri leaf extract at a concentration of 20, 40, and 60%. Conclusion: The Biduri leaf extract has antibacterial potential against S.mutans at concentrations of 20, 40, and 60%, and the best concentration in reducing the growth of S.mutans colonies is the concentration of 60%.
Keywords
Antibacterial, Calotropis gigantea, 0.2% chlorhexidine, colony counting, Streptococcus mutans
Keywords
Full Text:
PDFReferences
REFERENCES
Nicklisch, N., Oelze, V.M., Schierz, O., Meller, H., dan Alt, K.W. A healthier smile in the past dental caries and diet in early neolithic farming communities from central germany. Nutrients 2022(14) 1831.DOI: 10.3390/nu14091831
Komarudin, K., Apriasari, M.L., dan Azizah, A. Health behavior and dental carries in coastal community: literature review. ODONTO: Dent J; 2021.DOI: 10.30659/odj.9.2.279-289
Sedghi, L., DiMassa, V., Harrington, A., Lynch, S. V., dan Kapila, Y. L. The oral microbiome: role of key organisms and complex networks in oral health and disease. Periodontology 2000. 2021; 87(1): 107–131. DOI: 10.1111/prd.12393
Zhou, Xuedong dan Li, Yuqing. Atlas of oral microbiology from healthy microflora to disease. Springer; 2020.
Lemos, J. A., Palmer, S. R., Zeng, L., Wen, Z. T., Kajfasz, J. K., Freires, I. A., Abranches, J., & Brady, L. J. The biology of streptococcus mutans. Microbiology spectrum. 2018; 7(1). DOI: 10.1128/microbiolspec.GPP3-0051-2018
Matsumoto-Nakano M. Role of streptococcus mutans surface proteins for biofilm formation. The Japanese Dental Science Review. 2018; 54(1): 22–29. DOI:10.1016/j.jdsr.2017.08.002
Jothika, M., Vanajassun, P. P., & Someshwar, B. Effectiveness of probiotic, chlorhexidine and fluoride mouthwash against streptococcus mutans - randomized, single-blind, in vivo study. J of Int Society of Preventive & Community Dentistry. 2015; 5(Suppl 1): S44–S48.DOI:10.4103/2231-0762.156153
Kőhidai, Z., Takács, A., Lajkó, E., Géczi, Z., Pállinger, É., Láng, O., dan Kőhidai, L. The effects of mouthwashes in human gingiva epithelial progenitor (hgepp) cells. Clinical oral investigations. 2022; 26(6): 4559–4574. DOI: 10.1007/s00784-022-04422-z
Brookes ZLS, Bescos R, Belfield LA, Ali K, Roberts A. Current uses of chlorhexidine for management of oral disease: A Narrative Review J Dent. 2020; 103: 103497. doi: 10.1016/j.jdent.2020.103497.
Sisiana dan Misgiati. Mutu fisik sediaan salep ekstrak daun Biduri (calotropis gigantea) sebagai obat luka physical quality of Biduri leaf extract ointment (calotropis gigantea) as a wound medicine; 2021.
Alibasyah, Zulfan., Setya, Diana & Sinda, Maya. Aktivitas antibakteri ekstrak etanol 70% daun Biduri (calotropis gigantea) terhadap aggregatibacter actinomycetemcomitans atcc 29523. Cakradonya Dent J. 2020; 12: 56-63. DOI:10.24815/cdj.v12i1.17076.
Alibasyah, Z.M., Ningsih, D.S., Sunnati., R. Andayani & Ranggaswuni, N.P. Antibacterial effect of calotropis gigantea (l) extract on porphyromonas gingivalis. Journal of Biomimetics, Biomaterials and Biomedical Engineering. 2020; 48: 111 – 118.
Thomas, J., Kumar, M. Suresh., Wesely, E. G., Kumar, N. Vinod. Antimicrobial efficacy and phytochemical analysis of Calotropis gigantea (L.) R.Br. Int.J.Curr.Res.Biosci.Plantbiol. 2017; 4(9): 79-84.DOI:10.20546/ijcrbp.2017.409.012
Ahmad, Waseem. (2020). Preliminary phytochemical, antimicrobial and photochemical study of calotropis gigantea leaf extract. Current Chemistry Letters. 2020; 9: 105–112. DOIi: 10.5267/j.ccl.2019.10.001
Pudji A, Meilawaty Z, D suci AW,Setyaningsih S. Penapisan fitokimia dan kandungan flavonoid total tanaman Calotropis gigantea: studi eksperimental laboratoris. J Ked Gi. 2023; 35(2): 166-171. DOI: 10.24198/jkg.v35i2.46685
Hidayah, Nurul & Huda, Choirul & Tilarso, Dara. Uji aktivitas antibakteri fraksi daun Biduri (calotropis gigantea) terhadap staphylococcus aureus. JOPS (Journal Of Pharmacy and Science). 2021; 4: 40-45. 10.36341/jops.v4i1.1456.
Dewi, D. G. D. P., Mastra, N., & Jirna, I. N. Perbedaan zona hambat pertumbuhan Staphylococcus aureus pada berbagai konsentrasi ekstrak etanol daun Biduri Secara In Vitro. Meditory. 2018; 6(1): 39-45. https://doi.org/10.33992/m.v6i1.227
Mulqie, Lanny, Choesrina, Ratu & Suwendar, Suwendar & Mardliyani, Dieni. Potensi aktivitas antibakteri dari fraksi etil asetat daun jambu air [eugenia aqueum (burn f.) alston] terhadap sthaphylococcus aureus dan escherichia coli. J Ilm Farmasi Farmasyifa. 2019(2) 33-39.DOI:10.29313/jiff.v2i1.4230.
Kusumaningsih, Tuti., Sidarningsih., Putra, A. Andito., Aljunaid, M. Antibacterial differences effect between purple leaves (graptophyllum pictum (l) griff.) 70% and 96% ethanol extract against aggregatibacter actinomycetemcomittans bacteria. J of Int Dent and Med Research. 2021; 14(2): 519-524.
Oktaviani, R., Astuti, Pudji., Wahyukundari, Melok A. Aktivitas antibakteri ekstrak daun sirih merah (Piper crocatum) terhadap pertumbuhan Aggregatibacter actinomycetemcomitans. J Ked Gig Univ Padja. 2022; 34(1): 66-72.DOI: 10.24198/jkg.v34i1.34833
Barbieri, R., Coppo, E., Marchese, A., Daglia, M., Sobarzo-Sánchez, E., Nabavi, S. F., dan Nabavi, S. M. Phytochemicals for human disease: An update on plant-derived compounds antibacterial activity. Microbiological research. 2017; 196: 44–68.DOI: 10.1016/j.micres.2016.12.003
Terrones-Fernandez, I., Casino, P., López, A., Peiró, S., Ríos, S., Nardi-Ricart, A., García-Montoya, E., Asensio, D., Marqués, A. M., Castilla, R., Gamez-Montero, P. J., & Piqué, N. Improvement of the pour plate method by separate sterilization of agar and other medium components and reduction of the agar concentration. Microbiology spectrum. 2023; 11(1).DOI: 10.1128/spectrum.03161-22
Gutiérrez-Venegas, G., Gómez-Mora, J. A., Meraz-Rodríguez, M. A., Flores-Sánchez, M. A., & Ortiz-Miranda, L. F. Effect of flavonoids on antimicrobial activity of microorganisms present in dental plaque. Heliyon. 2019; 5(12). DOI: 10.1016%2Fj.heliyon.2019.e03013
Eren, M.M., Dikmen, B., Vatansever, C., Servi, H., Yegin, H.C., Ozan, G. Antimicrobial activity of sapindus mukorossi and saponaria officinalis extracts on streptococcus mutans and enterococcus faecalis. Ann. Med. Res. 2021; 28: 516–519.
Pambudi, A.Rilo., Wasiaturrahmah, Yusrinie & Aspriyanto, Didit. Antibacterial effectiveness of kecapi sentul extract (sandoricum koetjape merr.) against streptococcus mutans. ODONTO Dent J. 2021; 8(2).DOI: 10.30659/odj.8.2.1-10
Rabil, D., Satari, M.H., Chemiawan, E., & Gartika, M., The correlation between terpenoid of sarang semut (myrmecodia pendans) with mrna fructosyltranferase expression of streptococcus mutans biofilm atcc 25175. Odonto: Dent J; 2021.DOI: 10.30659/odj.8.1.86-93
Rosyadi, Afrian & Triatmoko, Bawon & Nugraha, Ari. Isolation of estuary soil fungi and screening antibacterial activity against staphylococcus aureus. Ind J of Pharma Science and Tech. 2022; 9(16).DOI: 10.24198/ijpst.v1i1.28834.
Kováč, J., Slobodníková, L., Trajčíková, E., Rendeková, K., Mučaji, P., Sychrová, A., & Bittner Fialová, S. Therapeutic potential of flavonoids and tannins in management of oral infectious diseases-a review. Molecules. 2022; 28(1): 158. DOI: 10.3390/molecules28010158
DOI: https://doi.org/10.24198/pjd.vol36no1.52850
Refbacks
- There are currently no refbacks.
Visitor Stat
Padjadjaran Journal of Dentistry is licensed under Creative Commons Attribution 4.0 International License