Cytotoxic Effects of Hibiscus surattensis L. Leaves Extracts on BSLT, MCF-7 and Hela Cells

Yuliet - Susanto

Abstrak


Hibiscus surattensis L. leaves have been known as a folk medicine. Several previous reports have shown several potential therapeutic effects, including antidiabetic, antibacterial, anti-diarrheal, analgesic, hepatoprotective, and antioxidant, but no toxicity study is similar to the current one. Therefore, it is necessary to investigate the cytotoxic effect of these commonly consumed medicinal leaves. This study aimed to investigate the cytotoxicity of Hibiscus surattensis L. leaves extract (HSL). The cytotoxic effect was determined through brine shrimp lethality bioassay (BSLT) and in vitro Presto Blue assay of MCF-7 and Hela cell.  HSL extract was obtained by maceration using 96% ethanol. The BSLT results showed that the HSL has LC50 value of 224.814 μg/mL. MTT assay results showed the IC50 value of the HSL extract on MCF-7 cells was 1419.83 μg/mL, and Hela cells were 2050.50 μg/mL. Based on the LC50 value that has been analyzed against HSL extract shows an LC50 value of less than 1000 μg/mL which can be concluded while having potential cytotoxic activity against experimental animal larvae of A. salina Leach. The results of the cytotoxic test with Presto Blue assay on two cancer cells used showed that the HSL extract was not toxic.


Kata Kunci


BSLT, Hibiscus surattensis L., cytotoxicity, MCF-7, Hela

Teks Lengkap:

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Referensi


Anoopa John L, Kannappan N MP. Evaluation of hepatoprotective activity in methanolic extract of aerial parts of Hibiscus surattensis. Res J Pharm Tech. 2020;13(10):4635-40.

Ellyn K. Damayanti, Ervizal A. M. Zuhud HMS. Kamus Penyakit dan Tumbuhan Obat Indonesia: (Etnofitomedika I). Sangat HM, editor. Indonesia: Yayasan Pustaka Obor Indonesia; 2000.

Yuliet, Sukandar EY, Atik N, Adnyana IK. Insulin secretion and repairing pancreatic tissue damage on diabetic mice treated with the extract and active fraction of Hibiscus surattensis L. leaves. J Pharm Pharmacogn Res. 2021;9(4):454-64.

Sultana S, Faruq A Al, Nahid-Al-Rashid, Nasim T, Ahsan MQ. In-vitro anti-inflammatory, anti-oxidant and in-vivo analgesic, antidiarrheal activities of fractional leaf extracts of Hibiscus surattensis. Eur J Pharm Med Res. 2018;5(4):167-73.

Akarca G. Composition and antibacterial effect on food borne pathogens of Hibiscus surrattensis L. calyces essential oil. Ind Crops Prod 2019;137(2):285-9.

Tiko GH, Medjigbodo A, Adamou R, Amoussa Amo, Djogbenou LS and Lagnika L. Scientific baseline information for the potential use of Hibiscus surattensis L against malaria: Phytochemistry and Biological Studies. J Drug Deliv Ther. 2020;10(5-s):127-35.

Kumala S, Sapitri DW. Phytochemical screening and toxicological Evaluation using brine shrimp lethality test (BSLT) of some fractions of Prasman leaves (Eupatorium triplinerve V) extract. Indones J Cancer Chemoprevention. 2011;2(1):193.

Boncler M, Rózalski M, Krajewska U, Podswdek A, Watala C. Comparison of PrestoBlue and MTT assays of cellular viability in the assessment of anti-proliferative effects of plant extracts on human endothelial cells. J Pharmacol Toxicol Methods. 2014;69(1):9-16.

Riskianto, Soemardji AA, Tan MI. Cytotoxic effects of Kirinyuh herb (Austroeupatorium inulaefolium (Kunth) R. d. King & H. Robinson) extracts and fractions on BSLT, MCF-7 Cells and T-47D Cells. Pharmacogn J. 2022;14(2):374-8.

Fareza MS, Choironi NA, Susilowati SS, Rini MP, Festihawa V, Fauzi ISN, et al. LC-MS/MS analysis and cytotoxic activity of extract and fractions of Calophyllum soulattri stembark. Indones J Pharm. 2021;32(3):356-64.

Lestari OA, Palupi NS, Setiyono A, Kusnandar F, Yuliana ND. In vitro antioxidant potential and phytochemical profiling of Melastoma malabathricum leaf water extract. Food Sci Technol. 2022;42.

Alves RT, Regasini LO, Funari CS, Young MCM, Rimoldi A, da Silva Bolzani V, et al. Trypanocidal activity of Brazilian plants against epimastigote forms from Y and Bolivia strains of Trypanosoma cruzi. Rev Bras Farmacogn. 2012;22(3):528-33.

Ueda JY, Tezuka Y, Banskota AH, Le Tran Q, Tran QK, Harimaya Y, et al. Antiproliferative activity of Vietnamese medicinal plants. Biol Pharm Bull. 2002;25(6):753-60.

Elsyana V, Bintang M, Priosoeryanto BP. Cytotoxicity and antiproliferative activity assay of clove mistletoe (Dendrophthoe pentandra (L.) Miq.) leaves extracts. Adv Pharmacol Sci. 2016;2016.

Chahardehi AM, Arsad H, Ismail NZ, Lim V. Low cytotoxicity, and antiproliferative activity on cancer cells, of the plant Senna alata (Fabaceae). Rev Biol Trop. 2021;69(1):317-30.

Syahputra G. Resazurin si indikator aktivitas sel. Bio Trends. 2015;6(2):26-8.

Tan, A. C., Konczak, I., Sze, D. M.Y., & Ramzan, I. Molecular pathways for cancer chemoprevention by dietary phytochemicals. Nutrition and Cancer.2011; 63(4), 495-505

Moorthy P, Rajan M, Sathyanarayanan S, Muniyandi K, Sivaraj D, Sasidharan SP, et al. Effect of different cooking methods of Hibiscus surratensis L. leaf vegetable on nutritional, anti-nutritional composition, and antioxidant activities. J Culin Sci Technol. 2018; 1: 13-28.

Pourahmad, J. , Salimi, A. , Seydi, E. Role of oxygen free radicals in cancer tevelopment and Treatment. In: Ahmad, R. editor. Free Radicals and Diseases [Internet]. London: IntechOpen; 2016 [cited 2022 Jul 26]. Available from: https://www.intechopen.com/chapters/51903 doi: 10.5772/64787

Anoopa John L, Kannappan N MP. Evaluation of hepatoprotective activity in methanolic extract of aerial parts of Hibiscus surattensis. Res J Pharm Tech. 2020;13(10):4635-40.

Ellyn K. Damayanti, Ervizal A. M. Zuhud HMS. Kamus Penyakit dan Tumbuhan Obat Indonesia: (Etnofitomedika I). Sangat HM, editor. Indonesia: Yayasan Pustaka Obor Indonesia; 2000.

Yuliet, Sukandar EY, Atik N, Adnyana IK. Insulin secretion and repairing pancreatic tissue damage on diabetic mice treated with the extract and active fraction of Hibiscus surattensis L. leaves. J Pharm Pharmacogn Res. 2021;9(4):454-64.

Sultana S, Faruq A Al, Nahid-Al-Rashid, Nasim T, Ahsan MQ. In-vitro anti-inflammatory, anti-oxidant and in-vivo analgesic, antidiarrheal activities of fractional leaf extracts of Hibiscus surattensis. Eur J Pharm Med Res. 2018;5(4):167-73.

Akarca G. Composition and antibacterial effect on food borne pathogens of Hibiscus surrattensis L. calyces essential oil. Ind Crops Prod 2019;137(2):285-9.

Tiko GH, Medjigbodo A, Adamou R, Amoussa Amo, Djogbenou LS and Lagnika L. Scientific baseline information for the potential use of Hibiscus surattensis L against malaria: Phytochemistry and Biological Studies. J Drug Deliv Ther. 2020;10(5-s):127-35.

Kumala S, Sapitri DW. Phytochemical screening and toxicological Evaluation using brine shrimp lethality test (BSLT) of some fractions of Prasman leaves (Eupatorium triplinerve V) extract. Indones J Cancer Chemoprevention. 2011;2(1):193.

Boncler M, Rózalski M, Krajewska U, Podswdek A, Watala C. Comparison of PrestoBlue and MTT assays of cellular viability in the assessment of anti-proliferative effects of plant extracts on human endothelial cells. J Pharmacol Toxicol Methods. 2014;69(1):9-16.

Riskianto, Soemardji AA, Tan MI. Cytotoxic effects of Kirinyuh herb (Austroeupatorium inulaefolium (Kunth) R. d. King & H. Robinson) extracts and fractions on BSLT, MCF-7 Cells and T-47D Cells. Pharmacogn J. 2022;14(2):374-8.

Fareza MS, Choironi NA, Susilowati SS, Rini MP, Festihawa V, Fauzi ISN, et al. LC-MS/MS analysis and cytotoxic activity of extract and fractions of Calophyllum soulattri stembark. Indones J Pharm. 2021;32(3):356-64.

Lestari OA, Palupi NS, Setiyono A, Kusnandar F, Yuliana ND. In vitro antioxidant potential and phytochemical profiling of Melastoma malabathricum leaf water extract. Food Sci Technol. 2022;42.

Alves RT, Regasini LO, Funari CS, Young MCM, Rimoldi A, da Silva Bolzani V, et al. Trypanocidal activity of Brazilian plants against epimastigote forms from Y and Bolivia strains of Trypanosoma cruzi. Rev Bras Farmacogn. 2012;22(3):528-33.

Ueda JY, Tezuka Y, Banskota AH, Le Tran Q, Tran QK, Harimaya Y, et al. Antiproliferative activity of Vietnamese medicinal plants. Biol Pharm Bull. 2002;25(6):753-60.

Elsyana V, Bintang M, Priosoeryanto BP. Cytotoxicity and antiproliferative activity assay of clove mistletoe (Dendrophthoe pentandra (L.) Miq.) leaves extracts. Adv Pharmacol Sci. 2016;2016.

Chahardehi AM, Arsad H, Ismail NZ, Lim V. Low cytotoxicity, and antiproliferative activity on cancer cells, of the plant Senna alata (Fabaceae). Rev Biol Trop. 2021;69(1):317-30.

Syahputra G. Resazurin si indikator aktivitas sel. Bio Trends. 2015;6(2):26-8.

Tan, A. C., Konczak, I., Sze, D. M.Y., & Ramzan, I. Molecular pathways for cancer chemoprevention by dietary phytochemicals. Nutrition and Cancer.2011; 63(4), 495-505

Moorthy P, Rajan M, Sathyanarayanan S, Muniyandi K, Sivaraj D, Sasidharan SP, et al. Effect of different cooking methods of Hibiscus surratensis L. leaf vegetable on nutritional, anti-nutritional composition, and antioxidant activities. J Culin Sci Technol. 2018; 1: 13-28.

Pourahmad, J. , Salimi, A. , Seydi, E. Role of oxygen free radicals in cancer tevelopment and Treatment. In: Ahmad, R. editor. Free Radicals and Diseases [Internet]. London: IntechOpen; 2016 [cited 2022 Jul 26]. Available from: https://www.intechopen.com/chapters/51903 doi: 10.5772/64787

Anoopa John L, Kannappan N MP. Evaluation of hepatoprotective activity in methanolic extract of aerial parts of Hibiscus surattensis. Res J Pharm Tech. 2020;13(10):4635-40.

Ellyn K. Damayanti, Ervizal A. M. Zuhud HMS. Kamus Penyakit dan Tumbuhan Obat Indonesia: (Etnofitomedika I). Sangat HM, editor. Indonesia: Yayasan Pustaka Obor Indonesia; 2000.

Yuliet, Sukandar EY, Atik N, Adnyana IK. Insulin secretion and repairing pancreatic tissue damage on diabetic mice treated with the extract and active fraction of Hibiscus surattensis L. leaves. J Pharm Pharmacogn Res. 2021;9(4):454-64.

Sultana S, Faruq A Al, Nahid-Al-Rashid, Nasim T, Ahsan MQ. In-vitro anti-inflammatory, anti-oxidant and in-vivo analgesic, antidiarrheal activities of fractional leaf extracts of Hibiscus surattensis. Eur J Pharm Med Res. 2018;5(4):167-73.

Akarca G. Composition and antibacterial effect on food borne pathogens of Hibiscus surrattensis L. calyces essential oil. Ind Crops Prod 2019;137(2):285-9.

Tiko GH, Medjigbodo A, Adamou R, Amoussa Amo, Djogbenou LS and Lagnika L. Scientific baseline information for the potential use of Hibiscus surattensis L against malaria: Phytochemistry and Biological Studies. J Drug Deliv Ther. 2020;10(5-s):127-35.

Kumala S, Sapitri DW. Phytochemical screening and toxicological Evaluation using brine shrimp lethality test (BSLT) of some fractions of Prasman leaves (Eupatorium triplinerve V) extract. Indones J Cancer Chemoprevention. 2011;2(1):193.

Boncler M, Rózalski M, Krajewska U, Podswdek A, Watala C. Comparison of PrestoBlue and MTT assays of cellular viability in the assessment of anti-proliferative effects of plant extracts on human endothelial cells. J Pharmacol Toxicol Methods. 2014;69(1):9-16.

Riskianto, Soemardji AA, Tan MI. Cytotoxic effects of Kirinyuh herb (Austroeupatorium inulaefolium (Kunth) R. d. King & H. Robinson) extracts and fractions on BSLT, MCF-7 Cells and T-47D Cells. Pharmacogn J. 2022;14(2):374-8.

Fareza MS, Choironi NA, Susilowati SS, Rini MP, Festihawa V, Fauzi ISN, et al. LC-MS/MS analysis and cytotoxic activity of extract and fractions of Calophyllum soulattri stembark. Indones J Pharm. 2021;32(3):356-64.

Lestari OA, Palupi NS, Setiyono A, Kusnandar F, Yuliana ND. In vitro antioxidant potential and phytochemical profiling of Melastoma malabathricum leaf water extract. Food Sci Technol. 2022;42.

Alves RT, Regasini LO, Funari CS, Young MCM, Rimoldi A, da Silva Bolzani V, et al. Trypanocidal activity of Brazilian plants against epimastigote forms from Y and Bolivia strains of Trypanosoma cruzi. Rev Bras Farmacogn. 2012;22(3):528-33.

Ueda JY, Tezuka Y, Banskota AH, Le Tran Q, Tran QK, Harimaya Y, et al. Antiproliferative activity of Vietnamese medicinal plants. Biol Pharm Bull. 2002;25(6):753-60.

Elsyana V, Bintang M, Priosoeryanto BP. Cytotoxicity and antiproliferative activity assay of clove mistletoe (Dendrophthoe pentandra (L.) Miq.) leaves extracts. Adv Pharmacol Sci. 2016;2016.

Chahardehi AM, Arsad H, Ismail NZ, Lim V. Low cytotoxicity, and antiproliferative activity on cancer cells, of the plant Senna alata (Fabaceae). Rev Biol Trop. 2021;69(1):317-30.

Syahputra G. Resazurin si indikator aktivitas sel. Bio Trends. 2015;6(2):26-8.

Tan, A. C., Konczak, I., Sze, D. M.Y., & Ramzan, I. Molecular pathways for cancer chemoprevention by dietary phytochemicals. Nutrition and Cancer.2011; 63(4), 495-505

Moorthy P, Rajan M, Sathyanarayanan S, Muniyandi K, Sivaraj D, Sasidharan SP, et al. Effect of different cooking methods of Hibiscus surratensis L. leaf vegetable on nutritional, anti-nutritional composition, and antioxidant activities. J Culin Sci Technol. 2018; 1: 13-28.




DOI: https://doi.org/10.24198/ijpst.v11i3.41525

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