Analysis of Virgin Coconut Oil (VCO) Purity Using UV-Vis Spectrophotometry Combined with PCA and HCA Chemometrics

Triyadi Hendra Wijaya; Rehana Rehana; Divaa' Rizqi Arisma; Nialiana Endah Endriastuti; Masita Wulandari Suryoputri

doi:10.24198/ijpst.v13i2.63230

Analysis of Virgin Coconut Oil (VCO) Purity Using UV-Vis Spectrophotometry Combined with PCA and HCA Chemometrics

Triyadi Hendra Wijaya, Rehana Rehana, Divaa' Rizqi Arisma, Nialiana Endah Endriastuti, Masita Wulandari Suryoputri

Abstrak

VCO is a vegetable oil with many health and cosmetic benefits, but it is relatively more expensive than other vegetable oils, making it vulnerable to adulteration. UV-Vis spectrophotometry, combined with chemometric techniques, was used to distinguish VCO from other oils. Soybean oil (SO) and sunflower oil (SFO) are the main adulterants of VCO. This study aimed to evaluate the ability of UV-Vis spectrophotometry combined with chemometrics to differentiate VCO from SO and SFO. Samples were measured for absorbance at wavelengths from 200 to 400 nm. The UV-Vis spectral data were further analyzed using chemometric methods, PCA, and HCA, to classify the samples. The PCA model successfully differentiated VCO, SO, and SFO in RStudio 4.4.2, with variance explained by PC1 (81.3%) and PC2 (17.1%). VCO samples are clearly separated from other samples, while SO and SFO appear close to each other due to their similar physicochemical properties. Mixed oil samples were grouped by composition. HCA analysis also showed a grouping pattern consistent with PCA results. Therefore, UV-Vis spectrophotometry, combined with chemometric techniques, can effectively distinguish VCO from SO and SFO.

Kata Kunci

Chemometrics; UV-Vis Spectrophotometry; Virgin Coconut Oil

Teks Lengkap:

PDF (English)

Referensi

Rohman A. Infrared spectroscopy for quantitative analysis and oil parameters of olive oil and virgin coconut oil: A review. Int J Food Prop. 2017;20(7):1447–56. https://doi.org/10.1080/10942912.2016.1213742

Widari NS, Saraswati R, Sutejo B. Optimization of Virgin Coconut Oil (VCO) Production with Diffuser Type Aeration Method. Eur J Eng Technol Res. 2021;6(4):139–43. https://doi.org/10.24018/ejeng.2021.6.4.2471

Nurminah M, Lubis LM, Munthe RM. Comparison of Virgin Coconut Oil (VCO) quality with fermentation and centrifugation methods from genjah and hybrid varieties of coconut based on Indonesian local environmental resources. IOP Conf Ser Earth Environ Sci. 2023;1241(1):1-8. http://dx.doi.org/10.1088/1755-1315/1241/1/012090

Pratiwi, I, Pardi, dan Yunus, M., Pemisahan asam laurat dari virgin coconut oil (VCO) dengan metode saponifikasi dan sonikasi. Proceeding Seminar Nasional Politeknik Negeri Lhokseumawe, vol. 2, no. 1, pp. 236-2399. 2018

De Luca M, Ioele G, Grande F, Occhiuzzi MA, Chieffallo M, Garofalo A, et al. Multivariate Curve Resolution Methodology Applied to the ATR-FTIR Data for Adulteration Assessment of Virgin Coconut Oil. Molecules. 2023;28(12):1-12. https://doi.org/10.3390/molecules28124661

Nuraisyah F, Praja ARI, Anwar N. Educational Assistance in Making Pure Cooking Oil for Coconut Farmers. Shihatuna : Jurnal Pengabdian Kesehatan Masyarakat. 2023;3(1).52-56. https://doi.org/10.30829/shihatuna.v3i1.13384

Amit, Jamwal R, Kumari S, Dhaulaniya AS, Balan B, Kelly S, et al. Utilizing ATR-FTIR spectroscopy combined with multivariate chemometric modeling for the swift detection of mustard oil adulteration in virgin coconut oil. Vib Spectrosc. 2020;109:103066.1-34.https://doi.org/10.1016/j.vibspec.2020.103066

Pizzo J, Galuch M, Santos P, Manin L, Zappielo C, Silva O, et al. Determination of Coconut Oil Adulteration with Soybean Oil by Direct Infusion Electrospray Ionization Mass Spectrometry. J Braz Chem Soc. 2019.

Ayu LR, Aliwarga L, Adisasmito S. Characterization of Fatty Acids and Antioxidant Activity of Moringa Seed Extract Oil. J Tek Media Pengembuhan Ilmu Dan Apl Tek. 2024;23(1):16–22. https://doi.org/10.55893/jt.vol23no1.568

Oca GM, Vera FID, Tapia AKG. Detection of refined coconut oil as adulterant in virgin coconut oil using UV-Vis spectroscopy coupled with PCA. Proceedings of the Samahang Pisika ng Pilipinas 41st Samahang Pisika ng Pilipinas Physics Conference. 2023; 1-4. https://dx.doi.org/10.1007/s12161-023-02506-4.

Godoy AC, Dos Santos PDS, Nakano AY, Bini RA, Siepmann DAB, Schneider R, et al. Analysis of Vegetable Oil from Different Suppliers by Chemometric Techniques to Ensure Correct Classification of Oil Sources to Deal with Counterfeiting. Food Anal Methods. 2020;13(5):1-10. https://link.springer.com/article/10.1007/s12161-020-01731-5

De Oliveira JB, Michels FS, Silva De Pádua Melo E, Nazário CED, Caires ARL, Gonçalves DA, et al. Data on mineral composition, fatty acids, oxidative stability, UV-VIS spectra and fluorescence emission of the Dersani® and Sunflower® oils used as a cicatrizing agent. Data Briefs. 2019;26:104427.1-9. https://doi.org/10.1016/j.dib.2019.104427

Ríos-Reina R, Azcarate SM. How Chemometrics Revives the UV-Vis Spectroscopy Applications as an Analytical Sensor for Spectralprint (Nontargeted) Analysis. Chemosensors. 2022. 22;11(1):8. https://doi.org/10.3390/chemosensors11010008

Mitsutake H, Gontijo LC, Santana FBD, Guimarães E, Rocha LLD, Borges Neto W. Use of Mass Spectrometry with Electrospray Ionization and Exploratory Analysis for Classification of Extra Virgin Olive Oil Adulterated with Vegetable Oils. Rev Virtual Quím. 2015;7(6):2180–2189. http://dx.doi.org/10.5935/1984-6835.20150129

Kucharska-Ambrożej K, Karpinska J. The application of spectroscopic techniques in combination with chemometrics for detection of adulteration of some herbs and spices. Microchem J. 2020;153:104-278. https://doi.org/10.1016/j.microc.2019.104278

Granato D, Santos JS, Escher GB, Ferreira BL, Maggio RM. Use of principal component analysis (PCA) and hierarchical cluster analysis (HCA) for multivariate association between bioactive compounds and functional properties in foods: A critical perspective. Trends Food Sci Technol. 2018 ;72:83–90. https://doi.org/10.1016/j.tifs.2017.12.006

Badan Standarisasi Nasional. (1992): Standar Nasional Indonesia No. 7381-2008, Minyak-Kelapa Virgin (VCO). http//:websisni.bsn.go.id.

Suloma A, El–Husseiny OM, A Zidan AENF, Mabroke RS. The efficiency of washout strategy on reducing the deposit of linoleic acid of tilapia fillet and viscera. Aquac Rep. 2022 ;23:1-8. https://doi.org/10.1016/j.aqrep.2022.101094

Rafi M, Jannah R, Heryanto R, Kautsar A, Septaningsih DA. UV-Vis spectroscopy and chemometrics as a tool for identification and discrimination of our Curcum species. International Food Research Journal. 2018; 25(2) : 643-648.

Jamwal R, Amit, Kumari S, Balan B, Kelly S, Cannavan A, et al. Rapid and non-destructive approach for the detection of fried mustard oil adulteration in pure mustard oil via ATR-FTIR spectroscopy-chemometrics. Spectrochim Acta A Mol Biomol Spectrosc. 2021;244:118822. 1-11. https://doi.org/10.1016/j.saa.2020.118822

Karim NA, Devarajan T, Ahmad A. Principal Component Analysis for Phenotypic Characterization of Sweet Potato (Ipomoea batatas (L.) Lam.) G Genotypes in Malaysia. Trends Sci. 2022. 9;19(12):4612.1-13. https://doi.org/10.48048/tis.2022.4612

Badan Standarisasi Nasional (1998) :. Standar Nasional Indonesia No. 01-4466-1998, Minyak Kedelai Sebagai Minyak Makan, http//:websisni.bsn.go.id.

Pratiwi RA, Nandiyanto ABD. How to Read and Interpret UV-VIS Spectrophotometric Results in Determining the Structure of Chemical Compounds. Indonesian Journal of Educational Research and Technology. 2020;2(1): 1–20.

https://doi.org/10.17509/ijert.v2i1.35171

Badan Standarisasi Nasional (1995) :. Standar Nasional Indonesia No.01-3720-1995, Minyak Biji Bunga Matahari, http//:websisni.bsn.go.id.

Jolliffe IT, Cadima J. Principal component analysis: a review and recent developments. Philos Trans R Soc Math Phys Eng Sci. 2016. 13;374(2065):20150202.1-16. https://doi.org/10.1098/rsta.2015.0202

Melvia S, Sri Gustini H, Agung N. Chemometric analysis of the Fourier Transform Infrared Spectrum Profile of Black Pepper Fruit (Piper Nigrum L.) From Several Regions in Indonesia. Pharmacoscript. 2023. 23;6(1):79–90. http://dx.doi.org/10.33263/BRIAC10.1721727

Brereton RG. Introduction to residuals and estimation by principal components analysis. J Chemom. 2022. Oct;36(10).1-7. https://doi.org/10.1002/cem.3407

Gusti N, Oktarina D, Elvia R, Nursa’adah E, Wardhana RW, Sudaryono A, et al. Facile Detection of Oil Adulteration using UV-Visible Spectroscopy Coupled with Chemometrics Analysis. Science and Technology Indonesia. 2021; 6(1), 1-5.

https://doi.org/10.26554/sti.2021.6.1.14-18

Hashem H, Almoshelhy RI, El-Waseif M, Magdy. Rapid Authentication of Extra Virgin Olive Oil using UV and FTIR Spectroscopy. Middle East Journal of Applied Sciences. 2020; 263-271. https://doi.org/10.36632/mejas/2020.10.2.25

Guntarti A, Pratiwi HK, Nurani LH, Gandjar IG. Authentication of lemongrass oil by gas chromatography-mass spectroscopy (GC-MS) combination chemometrics. Indonesian Journal of Pharmaceutical Science and Technology. 2022;9(3):174-80.

Sufriadi E, Idroes R, Meilina H, Munawar AA, Lelifajri, Indrayanto G. Partial Least Squares-Discriminant Analysis Classification for Patchouli Oil Adulteration Detection by Fourier Transform Infrared Spectroscopy in Combination with Chemometrics. ACS Omega, 2023;8(13):12348–61. https://doi.org/10.1021/acsomega.3c00080

Johnson JB, Thani PR, Naiker M. Detection of eucalyptus oil adulteration in Australian tea tree oil using UV–Vis and fluorescence spectroscopy. Talanta Open, 2022;6( 100169); 1-6.

https://doi.org/10.1016/j.talo.2022.100169

Messai H, Farman M, Sarraj-Laabidi A, Hammami-Semmar A, Semmar N. Chemometrics Methods for Specificity, Authenticity and Traceability Analysis of Olive Oils: Principles, Classifications and Applications. Foods. 2016;5(4):1-35. https://doi.org/10.3390/foods5040077.

DOI: https://doi.org/10.24198/ijpst.v13i2.63230