Effects of Platelet Rich Plasma and Hydroxyapatite Implants Derived from  Cuttlefish Bone (Sepia Sp) on Bone Generation and Osteogenic Activity

Sofia Sofia; Azhar Firdaus; Erwin Erwin; Zinatul Hayati

doi:10.24198/ijpst.v13i2.70155

Effects of Platelet Rich Plasma and Hydroxyapatite Implants Derived from Cuttlefish Bone (Sepia Sp) on Bone Generation and Osteogenic Activity

Sofia Sofia, Azhar Firdaus, Erwin Erwin, Zinatul Hayati

Abstrak

Bone defects require biocompatible biomaterials that support osteoconduction and tissue regeneration. Hydroxyapatite (HA) derived from cuttlebone (Sepia sp.) offers high calcium content and a porous structure with potential as a bone implant, while Platelet Rich Plasma (PRP) contains growth factors that enhance regeneration. This study aimed to evaluate the effects of PRP and cuttlebone HA on bone defect healing in rats using histopathological features. Nine male Wistar rats were divided into three groups: K1 (negative control), K2 (HA + PRP), and K3 (HA). Alveolar bone defects (5 mm in diameter) were surgically created, and treatments were applied accordingly. Samples were evaluated on day 30 using hematoxylin eosin staining and histomorphometric analysis. Parameters assessed included osteoblast, osteocyte, and osteoclast counts and neovascularization in the implant bone. One-way ANOVA revealed significant differences in osteoblast (p-value =0.000), osteoclast (p-value =0.033), and osteocyte (p-value =0.001) counts across groups. Kruskal-Wallis test showed significant difference in neovascularization (p-value =0.047). Histopathological examination demonstrated no necrosis and increased bone marrow proliferation in the K2 group. This study indicates that cuttlebone-derived hydroxyapatite holds potential as a regenerative implant, particularly when combined with PRP

Kata Kunci

Bone Defects; Cuttlefish Bone; Histopathology; Hydroxyapatite; Platelet Rich Plasma.

Teks Lengkap:

PDF (English)

Referensi

Lang NP, Bartold PM. Periodontal health. 2017;(May):9–16.

Wulandari P. The aging process and its relation to periodontal conditions. Explor Immunol. 2023 Jun;3:207–16.

Usui M, Onizuka S, Sato T, Kokabu S, Ariyoshi W, Nakashima K. Mechanism of alveolar bone destruction in periodontitis — Periodontal bacteria and inflammation. Jpn Dent Sci Rev. 2021 Nov;57:201–8.

Helmi MF, Huang H, Goodson JM, Hasturk H, Tavares M, Natto ZS. Prevalence of periodontitis and alveolar bone loss in a patient population at Harvard School of Dental Medicine. BMC Oral Health. 2019 Dec;19(1):254.

Soliman AM, Barreda DR. Acute Inflammation in Tissue Healing. 2023;

Yang N, Liu Y. The Role of the Immune Microenvironment in Bone Regeneration. Int J Med Sci. 2021;18(16):3697–707.

Khotib J. Acceleration of Bone Fracture Healing through the Use of Natural Bovine Hydroxyapatite Implant on Bone Defect Animal Model. 2019;55(3).

Gonz A. The Use of Shells of Marine Molluscs in Spanish Ethnomedicine : A Historical Approach and Present and Future Perspectives. 2023;

Battistella E, Mele S, Foltran I, Lesci IG, Roveri N, Sabatino P, et al. Cuttlefish Bone Scaffold for Tissue Engineering: A Novel Hydrothermal Transformation, Chemical-Physical, and Biological Characterization. J Appl Biomater Funct Mater. 2012 Jan;10(2):99–106.

Bacevich B, Smith R, Reihl A, Mazzocca A, Hutchinson I. Advances with Platelet-Rich Plasma for Bone Healing. Biol Targets Ther. 2024 Jan;Volume 18:29–59.

Article R. Platelet ‑ Rich Plasma : The Journey so Far ! 2020;685–92.

Jennes D, M. Philip L, K. Venugopal S, Anoop S, S. Devi S, et al. Radiographic evaluation of healing of critical size calvarial defect treated with silica-collagen-hydroxyapatite biocomposite graft and homologous platelet-rich plasma in rat model. J Vet Anim Sci. 2025;56(4):593–9.

Hamada C. Statistical analysis for toxicity studies. J Toxicol Pathol. 2018;31(1):15–22.

Chen J, Liu B, He J, Wei Y, He Y, Zhou H, et al. Multimodal mechanisms of platelet-rich plasma in bone defect repair : Angiogenesis , inflammation modulation , and metabolic regulation. Regen Ther. 2025;30(October):920–32.

Rodriguez IA, Kalaf EAG, Bowlin GL, Sell SA. Platelet-Rich Plasma in Bone Regeneration : Engineering the Delivery for Improved Clinical Efficacy. 2014;2014:392398.

Karakayal M. Effect of platelet-rich plasma on bone regenerate consolidation in distraction osteogenesis : An experimental study in rabbits. 2022;56(1):8–13.

Widiyanti P. Sintesis dan Karakterisasi Komposit Hidroksiapatit dari Tulang Sotong ( Sepia sp . ) -Kitosan untuk Kandidat Aplikasi Bone Filler.

Al-rawe RA, Al-rammahi HM, Cahyanto A, Ma A, Liew YM. Cuttlefish-Bone-Derived Biomaterials in Regenerative Medicine , Dentistry , and Tissue Engineering : A Systematic Review. 2024;1–40.

Febriani R, Reindah JJ, Aprilia NA. Efektivitas Nano-Hydroxyapatite Sebagai Upaya Kuratif Fracture Osteoporosis Melalui Mekanisme Osteokonduktif Bone Healing (Scaffold) : Tinjauan Sistematis. Jimki. 2025;11(2):31–44.

Supardi A, Nisa ZI, Hikmawati D. Synthesis of Nanohydroxyapatite from Cuttlefish Bone ( Sepia sp .) Using Milling Method. 2019;2019.

Aminatun A, Handayani DEF, Widiyanti P, Winarni D, Siswanto S. In vivo approach on femur bone regeneration of white rat (Rattus norvegicus) with the use of hydroxyapatite from cuttlefish bone (Sepia spp.) as bone filler. Vet World. 2019 Jun;12(6):809–16.

Mansouri K, Fattahian H, Mansouri N. The Role of Cuttlebone and Cuttlebone Derived Hydroxyapatite with Platelet Rich Plasma on Tibial Bone Defect Healing in Rabbit : An Experimental Study Tavşanlarda Mürekkepbalığı Kabuğu ve Kan Pulcuğundan Zengin Plazma İle Birlikte Mürekkep Balığı Kaynaklı. 2018;24(1):107–15.

Widarena R, Marlie H, Rubianto M, Setiawatie EM. Number of Osteoblasts and Osteoclasts in Combination Carbonate Hydroxyapatite, Platelet Rich Fibrin ( PRF ) and Antioxidant in Socket Rats Wistar After Tooth Extraction. 2021;4(1):15–8.

Setyoko B, Lufhf A, Verisandri A, Damayanti AT, Fitriana FA. Effect of carbonated hydroxyapatite synthesis from cuttlefish shells on orthodontic relapse prevention : in silico study. 2023;10(1):19–27.

Kennedy MM. Biomaterials-based approaches to mandibular tissue engineering : where we were , where we are , where we are going Background : Mandibular Defect Causes. 2025;(March).

Pang B, Xian J, Chen J, Ng L, Li M, Zhao G, et al. Cuttlefish Bone-Derived Calcium Phosphate Bioceramics Have Enhanced Osteogenic Properties. 2024;

Ashrin MN, Fitriana A, Nuriyanto AF, Ariani MD, Kridanto MJ. The potential effect of applying scaffold hydroxyapatite derived from Portunus pelagicus crab shells on osteonectin and VEGF-B expression. 2025;15(06):234–9.

Bacevich BM, David R, Smith J, Reihl AM, Mazzocca AD, Hutchinson ID. Biologics : Targets and Therapy Advances with Platelet-Rich Plasma for Bone Healing. 2024.

DOI: https://doi.org/10.24198/ijpst.v13i2.70155